recombinant mouse klotho protein  (R&D Systems)

Journal: Biochemical pharmacology

Article Title: Butyrate promotes kidney resilience through a coordinated kidney protective response in tubular cells.

doi: 10.1016/j.bcp.2024.116203

Figure Lengend Snippet: Fig. 3. Butyrate protected from experimental cisplatin-AKI. A) Plasma creatinine and B) plasma urea. C-E) Kidney mRNA expression of genes encoding the kidney protective factors Klotho (C), PGC-1α (D) and Nlrp6 (E) are higher in butyrate-treated mice with cisplatin-AKI than in WT mice with AKI. qRT-PCR. *p < 0.05, **p < 0.005, ***p < 0.0005 vs control mice or butyrate-treated cisplatin-AKI mice. F) Immunohistochemistry showing Klotho expression is preserved in butyrate-treated mice with cisplatin-AKI as compared to vehicle mice with AKI. Original magnification x20. Data expressed as mean ± SEM of n = 3–7 animals per group at 72 h.

Article Snippet: In a second cisplatin-AKI model, C57BL/6 mice were dosed intraperitoneally with recombinant mouse Klotho (rKlotho; 10 μg/kg, intraperitoneal injection; R&D System, Minneapolis, MN) or vehicle once a day starting one day before the cisplatin injection.

Techniques: Clinical Proteomics, Expressing, Quantitative RT-PCR, Control, Immunohistochemistry

Journal: Biochemical pharmacology

Article Title: Butyrate promotes kidney resilience through a coordinated kidney protective response in tubular cells.

doi: 10.1016/j.bcp.2024.116203

Figure Lengend Snippet: Fig. 6. Butyrate preserved nephroprotective factor expression in FA-AKI. A, B) Kidney expression for nephroprotective genes Klotho (A) and PGC-1α (B) was higher in butyrate-treated mice with AKI than in WT mice with AKI. Whole kidney qRT-PCR. C) Representative western blot and quantification *p < 0.05, **p < 0.005, ***p < 0.0005 vs control mice or butyrate-treated AKI mice. D) Immunohistochemistry showing Klotho and PGC-1α expression is preserved in butyrate-treated mice with AKI as compared to non-treated mice with AKI and localizes to tubular cells. Original magnification x20 and x40, respectively. Quantification is shown in Fig. 7. Data expressed as mean ± SEM of n = 3–7 animals per group at 72 h.

Article Snippet: In a second cisplatin-AKI model, C57BL/6 mice were dosed intraperitoneally with recombinant mouse Klotho (rKlotho; 10 μg/kg, intraperitoneal injection; R&D System, Minneapolis, MN) or vehicle once a day starting one day before the cisplatin injection.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Immunohistochemistry

Journal: Biochemical pharmacology

Article Title: Butyrate promotes kidney resilience through a coordinated kidney protective response in tubular cells.

doi: 10.1016/j.bcp.2024.116203

Figure Lengend Snippet: Fig. 7. Butyrate preserved nephroprotective factors expression in experimental FA-AKI. A, B) Quantification of immunohistochemistry Klotho and PGC-1α images shown in Fig. 4.D. C) Kidney expression of nephroprotective gene Nlrp6 is higher in butyrate-treated mice with AKI than in vehicle mice with AKI. qRT-PCR. Data expressed as mean ± SEM of n = 3–7 animals per group at 72 h. *p < 0.05, **p < 0.005, ***p < 0.0005 vs control mice or butyrate-treated FA-AKI mice.

Article Snippet: In a second cisplatin-AKI model, C57BL/6 mice were dosed intraperitoneally with recombinant mouse Klotho (rKlotho; 10 μg/kg, intraperitoneal injection; R&D System, Minneapolis, MN) or vehicle once a day starting one day before the cisplatin injection.

Techniques: Expressing, Immunohistochemistry, Quantitative RT-PCR, Control

Journal: Biochemical pharmacology

Article Title: Butyrate promotes kidney resilience through a coordinated kidney protective response in tubular cells.

doi: 10.1016/j.bcp.2024.116203

Figure Lengend Snippet: Fig. 8. Butyrate and the HDAC inhibitor trichostatin A (TSA) prevented TWEAK-induced upregulation of MCP1 and downregulation of kidney protective factors in cultured tubular cells. Cell extract mRNA expression for MCP-1 (A), Klotho (B), PGC-1α (C) and Nlrp6 (D). qRT-PCR. *p < 0.05 vs control or vehicle TWEAK.

Article Snippet: In a second cisplatin-AKI model, C57BL/6 mice were dosed intraperitoneally with recombinant mouse Klotho (rKlotho; 10 μg/kg, intraperitoneal injection; R&D System, Minneapolis, MN) or vehicle once a day starting one day before the cisplatin injection.

Techniques: Cell Culture, Expressing, Quantitative RT-PCR, Control

Journal: Biochemical pharmacology

Article Title: Butyrate promotes kidney resilience through a coordinated kidney protective response in tubular cells.

doi: 10.1016/j.bcp.2024.116203

Figure Lengend Snippet: Fig. 9. Butyrate prevented TWEAK, TNF-α and interferon-γ (TTI)-induced upregulation of MCP1 and downregulation of kidney protective factors in cultured tubular cells. Tubular cell mRNA expression for MCP-1 (A), Klotho (B), PGC-1α (C) and Nlrp6 (D). qRT-PCR. E) Klotho representative western blot and quantification. *p < 0.05, **p < 0.005, vs control or butyrate 5 mM- TTI.

Article Snippet: In a second cisplatin-AKI model, C57BL/6 mice were dosed intraperitoneally with recombinant mouse Klotho (rKlotho; 10 μg/kg, intraperitoneal injection; R&D System, Minneapolis, MN) or vehicle once a day starting one day before the cisplatin injection.

Techniques: Cell Culture, Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Biochemical pharmacology

Article Title: Butyrate promotes kidney resilience through a coordinated kidney protective response in tubular cells.

doi: 10.1016/j.bcp.2024.116203

Figure Lengend Snippet: Fig. 10. Butyrate prevented TWEAK-induced upregulation of MCP1 and downregulation of kidney protective factors in cultured tubular cells: dose-dependency. Tubular cell mRNA expression for MCP-1 (A), Klotho (B), PGC-1α (C) and Nlrp6 (D). qRT-PCR. *p < 0.05, **p < 0.005, vs control or butyrate- TWEAK.

Article Snippet: In a second cisplatin-AKI model, C57BL/6 mice were dosed intraperitoneally with recombinant mouse Klotho (rKlotho; 10 μg/kg, intraperitoneal injection; R&D System, Minneapolis, MN) or vehicle once a day starting one day before the cisplatin injection.

Techniques: Cell Culture, Expressing, Quantitative RT-PCR, Control

Journal: Biochemical pharmacology

Article Title: Butyrate promotes kidney resilience through a coordinated kidney protective response in tubular cells.

doi: 10.1016/j.bcp.2024.116203

Figure Lengend Snippet: Fig. 13. Conceptual representation of the impact of butyrate increasing kidney resilience by preserving kidney protective genes. A) Either the initial insult or inflammation promotes the activation of NFκB and histone deacetylation in tubular cells. As a result, genes encoding inflammatory mediators are transcribed, potentially amplifying inflammation and injury, while genes encoding kidney protective genes are suppressed. Through inhibition of NFκB activation and histone deacetylation, butyrate decreases inflammation and preserves the expression of kidney protective genes. B) To assess the relative contribution of the preservation of kidney protective genes, mice were administered recombinant Klotho, which reproduced the effect of butyrate increasing kidney resilience by preserving the expression of other kidney protective genes and decreasing kidney inflammation.

Article Snippet: In a second cisplatin-AKI model, C57BL/6 mice were dosed intraperitoneally with recombinant mouse Klotho (rKlotho; 10 μg/kg, intraperitoneal injection; R&D System, Minneapolis, MN) or vehicle once a day starting one day before the cisplatin injection.

Techniques: Preserving, Activation Assay, Inhibition, Expressing, Recombinant